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NEN Life Science nitrocellulose sheets
Nitrocellulose Sheets, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nitrocellulose+sheets/nitrocellulose+sheets/pm39201593-239-26-28
Average 90 stars, based on 1 article reviews
nitrocellulose sheets - by Bioz Stars, 2026-09
90/100 stars

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Electrophoresis:

Article Title: Oxidative stress mediates age-related hypertrophy of ligamentum flavum by inducing inflammation, fibrosis, and apoptosis through activating Akt and MAPK pathways.
Article Snippet: We centrifuged this solution at 12,000 rpm for 30 min and determined the protein concentration in the supernatant using protein assay dye (Bio-Rad Laboratories, Hercules, CA) with bovine serum albumin (BSA) as the standard. .. We loaded 50 μg of protein on 8% and 10% sodium dodecyl sulfate polyacrylamide gel for electrophoresis and then transferred it to nitrocellulose sheets (NEN Life Science Products, Inc, Boston, MA) in a transfer apparatus (Bio-Rad) running at 1.2 A for 2 h. After blocking the blots in 5% nonfat skim milk in TBST, we incubated the blots with primary antibodies, which were against target protein [GPX-1,iNOS], in 5% nonfat skim milk and then again with anti-rabbit IgG conjugated with alkaline phosphatase (dilution 1:5000; Jackson Immuno Research Laboratories, Inc, Philadelphia, PA). .. Immunoblots were developed using BCIP/NBT solution (Kirkegaard and Perry Laboratories, Inc, Baltimore, MD).

Article Title: Administration of N-Acetylcysteine to Regress the Fibrogenic and Proinflammatory Effects of Oxidative Stress in Hypertrophic Ligamentum Flavum Cells.
Article Snippet: The solution was centrifuged at 12,000 rpm for 10 minutes, and the protein concentration was measured using a protein assay dye (Bio-Rad Laboratories, Hercules, CA, USA), with bovine serum albumin as the standard. .. We added 8% and 10% sodium dodecyl sulfate polyacrylamide gel for electrophoresis and then transferred the samples to nitrocellulose sheets (NEN Life Science Products, Boston, MA, USA) in a transfer apparatus (Bio-Rad) running at 1.2 A for 2 h. After blocking the blots with 5% nonfat skimmed milk in Tween 20, we added primary antibodies against the target proteins (GPX-1, TGF-β, iNOS, β-actin, p38, p-p38, p65, p-p65, α-SMA, collagen I, and vimentin) and then again added anti-rabbit IgG conjugated with (a) (b) Figure 1: MRI scans of the lumbar spine. ..

Article Title: Tamarind xyloglucan attenuates dextran sodium sulfate induced ulcerative colitis: Role of antioxidation
Article Snippet: Ulcerative colitis (UC) is the leading chronic gastrointestinal disorder, if untreated can cause colon cancer.. We characterized tamarind xyloglucan (TXG) as an edible nano-fiber and investigated its role in UC mice.. TXG was an efficient free radical quencher and antioxidant in nature.

Article Title: Mucoadhesive role of tamarind xyloglucan on inflammation attenuates ulcerative colitis
Article Snippet: Western blot Analysis Cytoplasmic protein was extracted from the tissues by homogenizing in 200 μL of protein lysis buffer and then kept on ice for 30 min. After centrifugation (12000 rpm, 30 min), the protein concentration in the supernatant was determined by the protein assay dye (Bio-Rad Laboratory, Hercules, CA, USA) using BSA as the standard. .. Twenty to fifty μg protein was separated by electrophoresis in 10% SDS-PAGE, and transferred to nitrocellulose sheets (NEN Life Science Products, Inc, Boston, MA, USA) with a Western blot apparatus (Bio-Rad Laboratories, Hercules, CA, USA) run at 1.2 A for 2 h. After blocking in 5% non-fat skim milk, blots were incubated with rabbit anti-Muc1 (1:1000), rabbit anti-TLR4 (1:1000), rabbit anti-MyD88 (1:1000), rabbit anti-NF-κB (1:1000), rabbit anti-p-IκBα (1:1000)rabbit anti-GAPDH (1:1500), mouse anti-β-tubulin (1:1500) antibodies. .. Following washing with TBST, blots were incubated with anti-mice or rabbit IgG conjugated with alkaline phosphatase (dilution 1:3000) (Jackson ImmunoResearch Laboratories, Inc., Philadelphia, PA, USA).

Article Title: Daily sesame oil supplementation attenuates local renin-angiotensin system via inhibiting MAPK activation and oxidative stress in cardiac hypertrophy.
Article Snippet: Daily sesame oil supplementation attenuates local renin-angiotensin system via inhibiting MAPKs activation and oxidative stress in cardiac hypertrophy Chuan-Teng Liu MS, Ming-Yie Liu PhD PII: S0955-2863(16)30100-0 DOI: doi: 10.1016/j.jnutbio.2016.05.006 Reference: JNB 7593 To appear in: The Journal of Nutritional Biochemistry Received date: 23 December 2015 Revised date: 29 April 2016 Accepted date: 5 May 2016 Please cite this article as: Liu Chuan-Teng, Liu Ming-Yie, Daily sesame oil supplementation attenuates local renin-angiotensin system via inhibiting MAPKs activation and oxidative stress in cardiac hypertrophy, The Journal of Nutritional Biochemistry (2016), doi: 10.1016/j.jnutbio.2016.05.006 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Administration of N-Acetylcysteine to Regress the Fibrogenic and Proinflammatory Effects of Oxidative Stress in Hypertrophic Ligamentum Flavum Cells
Article Snippet: The solution was centrifuged at 12,000 rpm for 10 minutes, and the protein concentration was measured using a protein assay dye (Bio-Rad Laboratories, Hercules, CA, USA), with bovine serum albumin as the standard. .. We added 8% and 10% sodium dodecyl sulfate polyacrylamide gel for electrophoresis and then transferred the samples to nitrocellulose sheets (NEN Life Science Products, Boston, MA, USA) in a transfer apparatus (Bio-Rad) running at 1.2 A for 2 h. After blocking the blots with 5% nonfat skimmed milk in Tween 20, we added primary antibodies against the target proteins (GPX-1, TGF- β , iNOS, β -actin, p38, p-p38, p65, p-p65, α -SMA, collagen I, and vimentin) and then again added anti-rabbit IgG conjugated with alkaline phosphatase (dilution 1 : 5000; Jackson Immuno Research Laboratories, Philadelphia, PA, USA). .. Immunoblots were developed using 5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium solution (Kirkegaard and Perry Laboratories, Baltimore, MD, USA).

Blocking Assay:

Article Title: Oxidative stress mediates age-related hypertrophy of ligamentum flavum by inducing inflammation, fibrosis, and apoptosis through activating Akt and MAPK pathways.
Article Snippet: We centrifuged this solution at 12,000 rpm for 30 min and determined the protein concentration in the supernatant using protein assay dye (Bio-Rad Laboratories, Hercules, CA) with bovine serum albumin (BSA) as the standard. .. We loaded 50 μg of protein on 8% and 10% sodium dodecyl sulfate polyacrylamide gel for electrophoresis and then transferred it to nitrocellulose sheets (NEN Life Science Products, Inc, Boston, MA) in a transfer apparatus (Bio-Rad) running at 1.2 A for 2 h. After blocking the blots in 5% nonfat skim milk in TBST, we incubated the blots with primary antibodies, which were against target protein [GPX-1,iNOS], in 5% nonfat skim milk and then again with anti-rabbit IgG conjugated with alkaline phosphatase (dilution 1:5000; Jackson Immuno Research Laboratories, Inc, Philadelphia, PA). .. Immunoblots were developed using BCIP/NBT solution (Kirkegaard and Perry Laboratories, Inc, Baltimore, MD).

Article Title: Administration of N-Acetylcysteine to Regress the Fibrogenic and Proinflammatory Effects of Oxidative Stress in Hypertrophic Ligamentum Flavum Cells.
Article Snippet: The solution was centrifuged at 12,000 rpm for 10 minutes, and the protein concentration was measured using a protein assay dye (Bio-Rad Laboratories, Hercules, CA, USA), with bovine serum albumin as the standard. .. We added 8% and 10% sodium dodecyl sulfate polyacrylamide gel for electrophoresis and then transferred the samples to nitrocellulose sheets (NEN Life Science Products, Boston, MA, USA) in a transfer apparatus (Bio-Rad) running at 1.2 A for 2 h. After blocking the blots with 5% nonfat skimmed milk in Tween 20, we added primary antibodies against the target proteins (GPX-1, TGF-β, iNOS, β-actin, p38, p-p38, p65, p-p65, α-SMA, collagen I, and vimentin) and then again added anti-rabbit IgG conjugated with (a) (b) Figure 1: MRI scans of the lumbar spine. ..

Article Title: Tamarind xyloglucan attenuates dextran sodium sulfate induced ulcerative colitis: Role of antioxidation
Article Snippet: Ulcerative colitis (UC) is the leading chronic gastrointestinal disorder, if untreated can cause colon cancer.. We characterized tamarind xyloglucan (TXG) as an edible nano-fiber and investigated its role in UC mice.. TXG was an efficient free radical quencher and antioxidant in nature.

Article Title: Mucoadhesive role of tamarind xyloglucan on inflammation attenuates ulcerative colitis
Article Snippet: Western blot Analysis Cytoplasmic protein was extracted from the tissues by homogenizing in 200 μL of protein lysis buffer and then kept on ice for 30 min. After centrifugation (12000 rpm, 30 min), the protein concentration in the supernatant was determined by the protein assay dye (Bio-Rad Laboratory, Hercules, CA, USA) using BSA as the standard. .. Twenty to fifty μg protein was separated by electrophoresis in 10% SDS-PAGE, and transferred to nitrocellulose sheets (NEN Life Science Products, Inc, Boston, MA, USA) with a Western blot apparatus (Bio-Rad Laboratories, Hercules, CA, USA) run at 1.2 A for 2 h. After blocking in 5% non-fat skim milk, blots were incubated with rabbit anti-Muc1 (1:1000), rabbit anti-TLR4 (1:1000), rabbit anti-MyD88 (1:1000), rabbit anti-NF-κB (1:1000), rabbit anti-p-IκBα (1:1000)rabbit anti-GAPDH (1:1500), mouse anti-β-tubulin (1:1500) antibodies. .. Following washing with TBST, blots were incubated with anti-mice or rabbit IgG conjugated with alkaline phosphatase (dilution 1:3000) (Jackson ImmunoResearch Laboratories, Inc., Philadelphia, PA, USA).

Article Title: Administration of N-Acetylcysteine to Regress the Fibrogenic and Proinflammatory Effects of Oxidative Stress in Hypertrophic Ligamentum Flavum Cells
Article Snippet: The solution was centrifuged at 12,000 rpm for 10 minutes, and the protein concentration was measured using a protein assay dye (Bio-Rad Laboratories, Hercules, CA, USA), with bovine serum albumin as the standard. .. We added 8% and 10% sodium dodecyl sulfate polyacrylamide gel for electrophoresis and then transferred the samples to nitrocellulose sheets (NEN Life Science Products, Boston, MA, USA) in a transfer apparatus (Bio-Rad) running at 1.2 A for 2 h. After blocking the blots with 5% nonfat skimmed milk in Tween 20, we added primary antibodies against the target proteins (GPX-1, TGF- β , iNOS, β -actin, p38, p-p38, p65, p-p65, α -SMA, collagen I, and vimentin) and then again added anti-rabbit IgG conjugated with alkaline phosphatase (dilution 1 : 5000; Jackson Immuno Research Laboratories, Philadelphia, PA, USA). .. Immunoblots were developed using 5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium solution (Kirkegaard and Perry Laboratories, Baltimore, MD, USA).

Incubation:

Article Title: Oxidative stress mediates age-related hypertrophy of ligamentum flavum by inducing inflammation, fibrosis, and apoptosis through activating Akt and MAPK pathways.
Article Snippet: We centrifuged this solution at 12,000 rpm for 30 min and determined the protein concentration in the supernatant using protein assay dye (Bio-Rad Laboratories, Hercules, CA) with bovine serum albumin (BSA) as the standard. .. We loaded 50 μg of protein on 8% and 10% sodium dodecyl sulfate polyacrylamide gel for electrophoresis and then transferred it to nitrocellulose sheets (NEN Life Science Products, Inc, Boston, MA) in a transfer apparatus (Bio-Rad) running at 1.2 A for 2 h. After blocking the blots in 5% nonfat skim milk in TBST, we incubated the blots with primary antibodies, which were against target protein [GPX-1,iNOS], in 5% nonfat skim milk and then again with anti-rabbit IgG conjugated with alkaline phosphatase (dilution 1:5000; Jackson Immuno Research Laboratories, Inc, Philadelphia, PA). .. Immunoblots were developed using BCIP/NBT solution (Kirkegaard and Perry Laboratories, Inc, Baltimore, MD).

Article Title: Tamarind xyloglucan attenuates dextran sodium sulfate induced ulcerative colitis: Role of antioxidation
Article Snippet: Ulcerative colitis (UC) is the leading chronic gastrointestinal disorder, if untreated can cause colon cancer.. We characterized tamarind xyloglucan (TXG) as an edible nano-fiber and investigated its role in UC mice.. TXG was an efficient free radical quencher and antioxidant in nature.

Article Title: Mucoadhesive role of tamarind xyloglucan on inflammation attenuates ulcerative colitis
Article Snippet: Western blot Analysis Cytoplasmic protein was extracted from the tissues by homogenizing in 200 μL of protein lysis buffer and then kept on ice for 30 min. After centrifugation (12000 rpm, 30 min), the protein concentration in the supernatant was determined by the protein assay dye (Bio-Rad Laboratory, Hercules, CA, USA) using BSA as the standard. .. Twenty to fifty μg protein was separated by electrophoresis in 10% SDS-PAGE, and transferred to nitrocellulose sheets (NEN Life Science Products, Inc, Boston, MA, USA) with a Western blot apparatus (Bio-Rad Laboratories, Hercules, CA, USA) run at 1.2 A for 2 h. After blocking in 5% non-fat skim milk, blots were incubated with rabbit anti-Muc1 (1:1000), rabbit anti-TLR4 (1:1000), rabbit anti-MyD88 (1:1000), rabbit anti-NF-κB (1:1000), rabbit anti-p-IκBα (1:1000)rabbit anti-GAPDH (1:1500), mouse anti-β-tubulin (1:1500) antibodies. .. Following washing with TBST, blots were incubated with anti-mice or rabbit IgG conjugated with alkaline phosphatase (dilution 1:3000) (Jackson ImmunoResearch Laboratories, Inc., Philadelphia, PA, USA).

Article Title: Novel Aurora A Kinase Inhibitor Fangchinoline Enhances Cisplatin–DNA Adducts and Cisplatin Therapeutic Efficacy in OVCAR-3 Ovarian Cancer Cells-Derived Xenograft Model
Article Snippet: .. We loaded 50 μg of protein on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred it to nitrocellulose sheets (NEN Life Science Products, Inc., Boston, MA, USA) in a transfer apparatus (#1703930, Bio-Rad, Hercules, CA, USA) run at 1.2A for 3 h. After we blocked the blots in 5% nonfat skim milk in Tris-buffered saline Tween-20, the blots were incubated with Aurora A primary antibody (dilution 1:1000; I; #ab1287, Abcam, Cambridge, U.K.) or actin primary antibody (dilution 1:10000; #ab8226, Abcam) against target protein in 5% nonfat skim milk and then with secondary antibodies (dilution #14708, 1:5000 or #14709, 1:10000, respectively). .. After adding HRP substrates, the expression was detected using a charge-coupled device camera (ChemiDoc, Bio-Rad) and the software Image Lab 5.2.1 (Bio-Rad) [ ].

Magnetic Resonance Imaging:

Article Title: Administration of N-Acetylcysteine to Regress the Fibrogenic and Proinflammatory Effects of Oxidative Stress in Hypertrophic Ligamentum Flavum Cells.
Article Snippet: The solution was centrifuged at 12,000 rpm for 10 minutes, and the protein concentration was measured using a protein assay dye (Bio-Rad Laboratories, Hercules, CA, USA), with bovine serum albumin as the standard. .. We added 8% and 10% sodium dodecyl sulfate polyacrylamide gel for electrophoresis and then transferred the samples to nitrocellulose sheets (NEN Life Science Products, Boston, MA, USA) in a transfer apparatus (Bio-Rad) running at 1.2 A for 2 h. After blocking the blots with 5% nonfat skimmed milk in Tween 20, we added primary antibodies against the target proteins (GPX-1, TGF-β, iNOS, β-actin, p38, p-p38, p65, p-p65, α-SMA, collagen I, and vimentin) and then again added anti-rabbit IgG conjugated with (a) (b) Figure 1: MRI scans of the lumbar spine. ..

Western Blot:

Article Title: Tamarind xyloglucan attenuates dextran sodium sulfate induced ulcerative colitis: Role of antioxidation
Article Snippet: Ulcerative colitis (UC) is the leading chronic gastrointestinal disorder, if untreated can cause colon cancer.. We characterized tamarind xyloglucan (TXG) as an edible nano-fiber and investigated its role in UC mice.. TXG was an efficient free radical quencher and antioxidant in nature.

Article Title: Mucoadhesive role of tamarind xyloglucan on inflammation attenuates ulcerative colitis
Article Snippet: Western blot Analysis Cytoplasmic protein was extracted from the tissues by homogenizing in 200 μL of protein lysis buffer and then kept on ice for 30 min. After centrifugation (12000 rpm, 30 min), the protein concentration in the supernatant was determined by the protein assay dye (Bio-Rad Laboratory, Hercules, CA, USA) using BSA as the standard. .. Twenty to fifty μg protein was separated by electrophoresis in 10% SDS-PAGE, and transferred to nitrocellulose sheets (NEN Life Science Products, Inc, Boston, MA, USA) with a Western blot apparatus (Bio-Rad Laboratories, Hercules, CA, USA) run at 1.2 A for 2 h. After blocking in 5% non-fat skim milk, blots were incubated with rabbit anti-Muc1 (1:1000), rabbit anti-TLR4 (1:1000), rabbit anti-MyD88 (1:1000), rabbit anti-NF-κB (1:1000), rabbit anti-p-IκBα (1:1000)rabbit anti-GAPDH (1:1500), mouse anti-β-tubulin (1:1500) antibodies. .. Following washing with TBST, blots were incubated with anti-mice or rabbit IgG conjugated with alkaline phosphatase (dilution 1:3000) (Jackson ImmunoResearch Laboratories, Inc., Philadelphia, PA, USA).

Article Title: Daily sesame oil supplementation attenuates local renin-angiotensin system via inhibiting MAPK activation and oxidative stress in cardiac hypertrophy.
Article Snippet: Daily sesame oil supplementation attenuates local renin-angiotensin system via inhibiting MAPKs activation and oxidative stress in cardiac hypertrophy Chuan-Teng Liu MS, Ming-Yie Liu PhD PII: S0955-2863(16)30100-0 DOI: doi: 10.1016/j.jnutbio.2016.05.006 Reference: JNB 7593 To appear in: The Journal of Nutritional Biochemistry Received date: 23 December 2015 Revised date: 29 April 2016 Accepted date: 5 May 2016 Please cite this article as: Liu Chuan-Teng, Liu Ming-Yie, Daily sesame oil supplementation attenuates local renin-angiotensin system via inhibiting MAPKs activation and oxidative stress in cardiac hypertrophy, The Journal of Nutritional Biochemistry (2016), doi: 10.1016/j.jnutbio.2016.05.006 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Nucleic Acid Electrophoresis:

Article Title: Novel Aurora A Kinase Inhibitor Fangchinoline Enhances Cisplatin–DNA Adducts and Cisplatin Therapeutic Efficacy in OVCAR-3 Ovarian Cancer Cells-Derived Xenograft Model
Article Snippet: .. We loaded 50 μg of protein on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred it to nitrocellulose sheets (NEN Life Science Products, Inc., Boston, MA, USA) in a transfer apparatus (#1703930, Bio-Rad, Hercules, CA, USA) run at 1.2A for 3 h. After we blocked the blots in 5% nonfat skim milk in Tris-buffered saline Tween-20, the blots were incubated with Aurora A primary antibody (dilution 1:1000; I; #ab1287, Abcam, Cambridge, U.K.) or actin primary antibody (dilution 1:10000; #ab8226, Abcam) against target protein in 5% nonfat skim milk and then with secondary antibodies (dilution #14708, 1:5000 or #14709, 1:10000, respectively). .. After adding HRP substrates, the expression was detected using a charge-coupled device camera (ChemiDoc, Bio-Rad) and the software Image Lab 5.2.1 (Bio-Rad) [ ].

Saline:

Article Title: Novel Aurora A Kinase Inhibitor Fangchinoline Enhances Cisplatin–DNA Adducts and Cisplatin Therapeutic Efficacy in OVCAR-3 Ovarian Cancer Cells-Derived Xenograft Model
Article Snippet: .. We loaded 50 μg of protein on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred it to nitrocellulose sheets (NEN Life Science Products, Inc., Boston, MA, USA) in a transfer apparatus (#1703930, Bio-Rad, Hercules, CA, USA) run at 1.2A for 3 h. After we blocked the blots in 5% nonfat skim milk in Tris-buffered saline Tween-20, the blots were incubated with Aurora A primary antibody (dilution 1:1000; I; #ab1287, Abcam, Cambridge, U.K.) or actin primary antibody (dilution 1:10000; #ab8226, Abcam) against target protein in 5% nonfat skim milk and then with secondary antibodies (dilution #14708, 1:5000 or #14709, 1:10000, respectively). .. After adding HRP substrates, the expression was detected using a charge-coupled device camera (ChemiDoc, Bio-Rad) and the software Image Lab 5.2.1 (Bio-Rad) [ ].

Expressing:

Article Title: Daily sesame oil supplementation attenuates local renin-angiotensin system via inhibiting MAPK activation and oxidative stress in cardiac hypertrophy.
Article Snippet: Daily sesame oil supplementation attenuates local renin-angiotensin system via inhibiting MAPKs activation and oxidative stress in cardiac hypertrophy Chuan-Teng Liu MS, Ming-Yie Liu PhD PII: S0955-2863(16)30100-0 DOI: doi: 10.1016/j.jnutbio.2016.05.006 Reference: JNB 7593 To appear in: The Journal of Nutritional Biochemistry Received date: 23 December 2015 Revised date: 29 April 2016 Accepted date: 5 May 2016 Please cite this article as: Liu Chuan-Teng, Liu Ming-Yie, Daily sesame oil supplementation attenuates local renin-angiotensin system via inhibiting MAPKs activation and oxidative stress in cardiac hypertrophy, The Journal of Nutritional Biochemistry (2016), doi: 10.1016/j.jnutbio.2016.05.006 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Molecular Weight:

Article Title: Inhibiting CD44-ICD Attenuates LPS-Induced Initiation of Hepatic Inflammation in Septic Mice.
Article Snippet: .. Fifty micrograms of proteins were loaded on 8–15% sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE), depending on the molecular weight of the target protein, and then transferred to nitrocellulose sheets (NEN Life Science Products, Inc., Boston, MA, USA). .. After blocking, the blots were incubated with iNOS (1:1000; BD Bioscience, Franklin Lakes, NJ, USA, 610432), p-IKK α/β (1:1000; Genetex, Washington, DC, USA, GTX9039), p-IκB (1:1000; Genetex, GTX00967), nuclear NF-κB (1:500; Genetex, GTX102090), IL-1β (1:1000, Santacruz, Dallas, TX, USA, sc-127420), CD44 (1:500; proteintech, 15675-1-AP), nuclear CD44-ICD (1:500; Cosmobio, Tokyo, Japan, #KAL-K0604), GAPDH (1:5000; abcam, ab9485), β tubulin (1:1000; abcam, ab6046), and Lamin B1 (1:1000; abcam, ab16048) in 5% nonfat skim milk.



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